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KMID : 0364219920350010017
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Korean Journal of Zoology
1992 Volume.35 No. 1 p.17 ~ p.22
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Isolation and Partial Chemical Characterization of the Yolk Proteins from Drosophila sp. (robusta species group)
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Kim Se-Jae
Kim Gi-Ok
Oh Moon-You
Lee Chung-Choo
Kim Kyung-Jin
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Abstract
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The three yolk polypeptides have been isolated and partially characterized. Their molecular weights of YPI, YP2, and YP3 were 48, 000, 47, 000, and 46, 000, respectivelv, as judged by SDS-polyacrvlamide gel electrophoresis. They have different digestion products upon in situ peptide mapping by limited proteolysis. Two-dimensional gel electrophoresis showed that their isoelectric points were heterogeneous from 5.92 to 6.54. And thew showed three different antigen-antibody reactions when each polvpeptides is reacted with antisera made to a mixture of all of three. These data reported here indicate that the yolk proteins are consisted of distinctive polypeptides in Drosophlla sp. (robusta species group).
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KEYWORD
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Drospphila yolk proteins, Purification, Partial chemical characterizion
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